7/6/2023 0 Comments Smartscope fold microscope![]() As of October, the company had shipped 510,000, according to Prakash. Prakash and his former graduate student Jim Cybulski are the cofounders, and they’ve set a goal of distributing a million Foldscopes by the end of 2018. ![]() Foldscope is now backed by a startup company, Foldscope Instruments of Palo Alto, Calif. Prakash first conceived of Foldscope in 2011 while traveling in Thailand. This “Paperfuge” has applications in both field work and developing countries. Prakash is also the designer of a 20-cent whirligig biomedical centrifuge, made only of paper and string, that separates pure plasma from whole blood in under 1.5 minutes and separates malaria parasites in under 15 minutes. And instead of an eyepiece, you can use your smartphone’s camera, which can be magnetically docked to the Foldscope thanks to adhesive magnets. With such clever design hacks, the cost of the parts needed to make one Foldscope is now less than one U.S. Drawing inspiration from DNA-folding studies, space telescope design, and much more, Prakash says he’s able to achieve optical alignments in this folded-cardboard device of as little as 20 micrometers. The Foldscope uses origami to make a stable cardboard microscope body and precision-manufactured glass beads for a lens. Thanks to Manu Prakash, an assistant professor of bioengineering at Stanford University, in California, there are now more than a half-million “Foldscopes” out in the world. ![]() At least its design hadn’t changed until a Stanford engineer started playing with innovative microscopy ideas in 2011. The basic tabletop microscope hasn’t changed much in fundamental design in nearly 100 years. arXiv.Mobile Microscope: The deluxe Foldscope kits includes a magnetic smartphone coupler, materials to make slides, and an LED light to illuminate samples. CryoRL: Reinforcement Learning Enables Efficient Cryo-EM Data Collection. A novel processive mechanism for DNA synthesis revealed by structure, modeling and mutagenesis of the accessory subunit of human mitochondrial DNA polymerase. doi: 10.1016/j.jsb.2018.07.015.įan L, Kim S, Farr CL, Schaefer KT, Randolph KM, Tainer JA, Kaguni LS. High resolution single particle cryo-electron microscopy using beam-image shift. Ĭheng A, Eng ET, Alink L, Rice WJ, Jordan KD, Kim LY, Potter CS, Carragher B. īouvette J, Huang Q, Riccio AA, Copeland WC, Bartesaghi A, Borgnia MJ. Our automated tool for systematic evaluation of specimens streamlines structure determination and lowers the barrier of adoption for cryo-EM.Īutomation cryo-electron microscopy deep learning human machine learning molecular biophysics object recognition software platform structural biology.īouvette J, Huang Q, Riccio AA, Copeland WC, Bartesaghi A, Borgnia MJ. Manual annotations can be used to re-train the feature recognition models, leading to improvements in performance. A web interface provides remote control over the automated operation of the microscope in real time and access to images and annotation tools. SmartScope employs deep-learning-based object detection to identify and classify features suitable for imaging, allowing it to perform thorough specimen screening in a fully automated manner. Here, we present SmartScope, the first framework to streamline, standardize, and automate specimen evaluation in cryo-EM. ![]() While automation has significantly increased the speed of data collection, specimens are still screened manually, a laborious and subjective task that often determines the success of a project. Finding the conditions to stabilize a macromolecular target for imaging remains the most critical barrier to determining its structure by cryo-electron microscopy (cryo-EM). ![]()
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